Human herpesvirus detection and species identification with PCR-RFLP and ELISA: a comparative study
نویسندگان
چکیده
Methods: A pair of primers was designed to amplify herpes simplex virus type 1 and 2 (HSV-1/-2), EpsteinBarr virus (EBV) and cytomegalovirus (CMV). At last, we used the PCR-RFLP technique to differentiate four different herpesviruses. Meanwhile, 75 clinical blood specimens from infants of suspected viral infection and 38 blood specimens from healthy children were evaluated for herpesviruses DNA by PCR-RFLP or virus-specific IgM antibody detection by ELISA.
منابع مشابه
Detection and discrimination of two Brucella species by multiplex real-time PCR and high-resolution melt analysis curve from human blood and comparison of results using RFLP
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